Journal: Oncology Letters

Article Title: lncRNAs are novel biomarkers for differentiating between cisplatin-resistant and cisplatin-sensitive ovarian cancer

doi: 10.3892/ol.2018.8433

Figure Lengend Snippet: Differential lncRNA expression profiles between tissues of cisplatin-resistant and cisplatin-sensitive ovarian cancer. The lncRNA microarray demonstrated the differences between lncRNA expression in cisplatin-resistant and cisplatin-sensitive ovarian cancer through (A) hot-spot and (B) cluster map analysis. (C) Based on the association of the nearby coding genes, the differentially expressed lncRNAs were classified into certain types, including 312 natural antisense, 216 intronic antisense, 114 intron sense-overlapping, 673 intergenic, 201 exon sense-overlapping and 72 bidirectional lncRNA. lncRNA, long non-coding RNA.

Article Snippet: Following washing, the arrays were scanned using the Agilent lncRNA Microarray Scanner (Agilent Technologies, Inc.), and the Agilent Feature Extraction software version 11.0.1.1 (Agilent Technologies, Inc.) was used for microarray probe signal data collection.

Techniques: Expressing, Microarray

Journal: Journal of Cellular and Molecular Medicine

Article Title: Unveiling the role of the KLF4/Lnc18q22.2/ULBP3 axis in the tumorigenesis and immune escape of hepatocellular carcinoma under hypoxic condition

doi: 10.1111/jcmm.18411

Figure Lengend Snippet: Lnc18q22.2 is upregulated in hypoxia induced HCC cells. (A, B) SMMC7721 and Hep3B cells were cultured under normoxic and hypoxic conditions and then subjected to lncRNA microarray analysis. (C) The top eight significantly upregulated lncRNAs were listed as indicated. (D, E) HCC cells were subjected to treatment under hypoxic/normoxic, Lnc18q22.2 was measured at different time poinst as indicated. (F, G) HCC cells were exposed to 100 μM CoCl2 with different concentrations 8 h, Lnc18q22.2 was measured as indicated. (H, I) the subcellular distribution of Lnc18q22.2 in HCC cells was validated. (J, K) SMMC7721 cells underwent transfection with si‐NC and si‐HIF‐1α. Following a 24‐h transfection period, cells were cultured under either normoxic or hypoxic conditions for an additional 24 h. The expression levels of HIF‐1α were assessed through western blot analysis, while the levels of Lnc18q22.2 expression were determined using qRT‐PCR. (L, M) SMMC7721 cells were transfected with pcDNA3.1‐HIF‐1α. After a 24‐h transfection period, the expression level of HIF‐1α was assessed through western blot analysis. Concurrently, the levels of Lnc18q22.2 expression were examined using qRT‐PCR. The results were presented as mean ± SD with a sample size of n = 3.

Article Snippet: The microarray was subsequently analysed using the Arraystar lncRNA Microarray and scanned with the Agilent G2505C Scanner.

Techniques: Cell Culture, Microarray, Transfection, Expressing, Western Blot, Quantitative RT-PCR

Journal: International Journal of Women's Health

Article Title: Antiviral Therapy-Induced Changes in Long Non-Coding RNA Expression Profiles in Umbilical Cord Blood and Placental Tissues of Hepatitis B Virus-Infected Pregnant Women

doi: 10.2147/IJWH.S511524

Figure Lengend Snippet: LncRNA microarray analysis of the effect of antiviral therapy on lncRNA expression profiles in umbilical cord blood after delivery. ( a ) In this study, umbilical cord blood was collected from 6 pregnant women with high HBV viral load but no intrauterine infection, 3 of whom did not receive antiviral therapy (p1) and 3 of whom received antiviral therapy (p2). LncRNA expression in serum P1 and P2 was detected by the microarray. Volcano map showed the effect of antiviral therapy on lncRNA expression profiles in umbilical cord blood. ( b ) Heat map showed the top 10 significantly upregulated and downregulated lncRNAs in umbilical cord blood after antiviral therapy.

Article Snippet: After cleaning, the Arraystar Human LncRNA Microarray (V5) was scanned with the Agilent microarray scanner (Agilent Technologies Inc).

Techniques: Microarray, Expressing, Infection

Journal: International Journal of Women's Health

Article Title: Antiviral Therapy-Induced Changes in Long Non-Coding RNA Expression Profiles in Umbilical Cord Blood and Placental Tissues of Hepatitis B Virus-Infected Pregnant Women

doi: 10.2147/IJWH.S511524

Figure Lengend Snippet: GSEA pathway enrichment analysis. ( a–d ) GSEA pathway enrichment analysis revealed 4 antiviral signaling pathways activated by lncRNA in umbilical cord blood.

Article Snippet: After cleaning, the Arraystar Human LncRNA Microarray (V5) was scanned with the Agilent microarray scanner (Agilent Technologies Inc).

Techniques: Protein-Protein interactions

Journal: International Journal of Women's Health

Article Title: Antiviral Therapy-Induced Changes in Long Non-Coding RNA Expression Profiles in Umbilical Cord Blood and Placental Tissues of Hepatitis B Virus-Infected Pregnant Women

doi: 10.2147/IJWH.S511524

Figure Lengend Snippet: LncRNA microarray analysis of the effect of antiviral therapy on lncRNA expression profiles in placenta tissues. ( a ) Placenta tissues were collected from 6 postnatal women with high hepatitis B viral load but no intrauterine infection, 3 of whom did not receive antiviral therapy (U1) and 3 of whom received antiviral therapy (U2). LncRNA microarray was used to detect lncRNA expression in U1 and U2 placental tissues. Volcano map showed the effect of antiviral therapy on lncRNA expression profiles in placenta tissues. ( b ) Heat map showed the top 10 significantly upregulated and downregulated lncRNAs in placental tissues after antiviral therapy.

Article Snippet: After cleaning, the Arraystar Human LncRNA Microarray (V5) was scanned with the Agilent microarray scanner (Agilent Technologies Inc).

Techniques: Microarray, Expressing, Infection

Journal: International Journal of Women's Health

Article Title: Antiviral Therapy-Induced Changes in Long Non-Coding RNA Expression Profiles in Umbilical Cord Blood and Placental Tissues of Hepatitis B Virus-Infected Pregnant Women

doi: 10.2147/IJWH.S511524

Figure Lengend Snippet: GSEA pathway enrichment analysis. ( a–c ) GSEA pathway enrichment analysis revealed 3 signaling pathways activated by lncRNA in placenta tissues.

Article Snippet: After cleaning, the Arraystar Human LncRNA Microarray (V5) was scanned with the Agilent microarray scanner (Agilent Technologies Inc).

Techniques: Protein-Protein interactions

Journal: Cancer Cell International

Article Title: Macrophage polarization-associated lnc-Ma301 interacts with caprin-1 to inhibit hepatocellular carcinoma metastasis through the Akt/Erk1 pathway

doi: 10.1186/s12935-021-02133-1

Figure Lengend Snippet: Microarray analysis of long non-coding RNAs (lncRNAs) and mRNAs during polarization from M2 to M1 macrophages. A CD68 and CD163 immunohistochemical staining in hepatocellular carcinoma (HCC) tissues and corresponding adjacent normal tissues (n = 30). The images represent the distributions of M1 and M2 cells inHCC tissues and adjacent normal tissues. Pictures were taken at 200× magnification. B M2 and M1 phenotype identification. U937 cells gave rise to M2 phenotype macrophages after stimulation with PMA, IL-4, and IL-13, which were polarized into M1 phenotype macrophages by LPS and IFN-γ stimulation. Western blotting, ELISA and qRT-PCR were used to identify the M2 and M1 phenotypes. β-actin was used as a reference for Western blotting. Anti-Arg-1 (40 KD) antibody was used as a marker of the M2 phenotype and iNOS (130 KD) antibody as a marker of the M1 phenotype. TGF-β, IL-10, and IL-12 were also used as markers to identify M2 and M1 macrophages. C Workflow for lncRNA analysis. D Clustering and pairwise comparison of lncRNAs differentially expressed between M1 and M2 cells. E Distribution of six types of lncRNAs as well as down- and up-regulated lncRNAs and mRNAs. F qRT-PCR validation of the selected genes from the microarray data. G Pearson correlation analysis to assess relationships between microarray and qRT-PCR results

Article Snippet: Finally, the hybridized arrays were washed, fixed, and scanned using the Agilent DNA Microarray Scanner (G2565BA; Agilent). lncRNA expression profiles were analyzed using the SBC Human (4 × 180 K) lncRNA Microarray version 3.0 (Arraystar LncRNA microarray, USA).

Techniques: Microarray, Immunohistochemical staining, Staining, Western Blot, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Marker